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IHC testing of human colorectal carcinoma with anti-EpCAM antibody (clone EPM17-2).
IHC testing of human colorectal carcinoma with anti-EpCAM antibody (clone EPM17-2).

EpCAM Antibody [EPM17-2]

CATALOG NUMBER: 34-061

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Human partial recombinant protein (extracellular portion) was used as the immunogen for this anti-EpCAM antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western blot: 1-2 ug/ml
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-EpCAM antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10mM Citrate Buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
EPCAM
Additional Names:
Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Ncbi Gene Id Number:
4072
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-061

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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