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43-574 (0.03ug/ml) staining of CACO-2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-574 (0.03ug/ml) staining of CACO-2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EPCAM / TROP1 Antibody

CATALOG NUMBER: 43-574

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QKEITTRYQLDPK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell line CACO-2 (calculated MW of 34.9kDa according to NP_002345.2). Recommended concentration: 0.03-0.1ug/ml.
Predicted Molecular Weight:
Approx 37kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EPCAM / TROP1
Additional Names:
EPCAM, epithelial cell adhesion molecule, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, adenocarcinoma-associated antigen, cell surface glycoprotein Trop-1, epithelial glycoprotein 314, human epithelial glycoprot
Protein Accession Number:
NP_002345.2
Protein Gi Number:
160266056
Ncbi Gene Id Number:
4072
Background Reference 1:
Wu CJ, Mannan P, Lu M, Udey MC.Epithelial cell adhesion molecule (EpCAM) regulates claudin dynamics and tight junctions.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-574

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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