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Western blot analysis of lysates from Mouse spleen, using EPCR/CD201 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.
Flow cytometry: 1X10^6 Jurkat cells(low Expression,left) and A549 (right) were surface-stained with CD201/EPCR Rabbit mAb(2 μg/mL,orange line) or PSFlo 647 Rabbit IgG isotype control,followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb (1:200 dilution) staining.Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 A549 cells were surface-stained with PSFlo 647 Rabbit IgG isotype control or CD201/EPCR Rabbit mAb(2 μg/mL,right).
Western blot analysis of lysates from Mouse spleen, using EPCR/CD201 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.

EPCR/CD201 Rabbit Recombinant Antibody (82-908)

CATALOG NUMBER: 82-908

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB
Application Note:
WB,1:500 - 1:1000, FC,1:100 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
26kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PROCR
Additional Names:
PROCR; CCCA; CCD41; EPCR; protein C receptor; EPCR/CD201
Protein Accession Number:
Q9UNN8
Ncbi Gene Id Number:
10544
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-908

List Size:
100 uL

List Price:

$477.00

Datasheet
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