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Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody. The lane on the right is treated with the antigen-specific peptide.

EPHA 2/3/4 (phospho Tyr588/596) Antibody

CATALOG NUMBER: 79-811

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
EPHA 2/3/4 (phospho Tyr588/596) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y (p)-V-R)/ (R-T-Y (p)-V-D) derived from Human EPHA2/3/4.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
The antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596
Predicted Molecular Weight:
130 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EPHA2, EPHA3, EPHA4
Additional Names:
ECK, MPK-5, SEK2, kinase EphA2
Protein Accession Number:
NP_004422.2
Protein Gi Number:
32967311
Ncbi Gene Id Number:
1969;2042;2043
Background:
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.
Background Reference 1:
Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990).
Background Reference 2:
Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005).
Background Reference 3:
Zhuang G., J. Biol. Chem. 282:2683-2694(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-811

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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