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Western blot analysis of EphA10 in 293 cell lysate with EphA10 antibody at (A) 1 μg/mL and (B) 2 μg/mL.
Western blot analysis of EphA10 in 293 cell lysate with EphA10 antibody at (A) 1 μg/mL and (B) 2 μg/mL.
Trial Size

EphA10 Antibody

CATALOG NUMBER: 5253

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
EphA10 antibody was raised against a 14 amino acid synthetic peptide of near the amino terminus of human EphA10.
The immunogen is located within amino acids 120 - 170 of EphA10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EphA10 antibody can be used for detection of EphA10 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Purification:
EphA10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
EphA10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
EphA10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EPHA10
Additional Names:
EphA10 Antibody: Ephrin type-A receptor 10
Protein Accession Number:
NP_001092909
Protein Gi Number:
150456460
Ncbi Gene Id Number:
284656
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EphA10 Antibody: Eph receptors, the largest subfamily of receptor tyrosine kinases (RTKs), and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment, shape, and mobility of neuronal and endothelial cells in central nervous system function and in development. Eph receptors can be divided into two subgroups: EphA and EphB. In mammals, the EphA class consists of eight members (EphA 1-7 and 10) that in general bind to ephrin-A members linked to the cell membrane through a glycosylphosphatidylinositol linkage. The EphB class consists of six members (EphB 1-6) that in general bind ephrin-B members that transverse the cell membrane. The Ephrin / EPH signaling pathway networks with the WNT signaling pathway during embryogenesis, tissue regeneration, and carcinogenesis. Recent studies show that Eph/EFN might be relevant in normal B-cell biology and could represent new potential prognostic markers and therapeutic targets for CLL.
Background Reference 1:
Flanagan JG and Vanderhaeghen P. The ephrins and Eph receptors in neural development. Annu. Rev. Neurosci.1998;.21:309-45.
Background Reference 2:
Frisen J, Holmberg J, and Barbacid M. Ephrins and their Eph receptors: multitalented directors of embryonic development. EMBO J.1999; 18:5159-65.
Background Reference 3:
Eph Nomenclature Committee. Unified nomenclature for Eph family receptors and their ligands, the ephrins. Cell1997; 90:403-4.
Background Reference 4:
Holder N and Klein R. Eph receptors and ephrins: effectors of morphogenesis, Development1999; 126:2033-44.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5253

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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