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Human EphA4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human EphA4, His Tag at 5 ug/mL (100 uL/well) can bind Human Ephrin-A4, Fc Tag with a linear range of 0.6-5 ng/mL (QC tested).
Human EphA4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

EphA4 Recombinant Protein

CATALOG NUMBER: 11-257

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Val 20 - Thr 547
Fusion Tag:
His Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
60.3 kDa
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
EPHA4
Additional Names:
EphA4,HEK8,SEK,TYRO1,EK8
Protein Accession Number:
NP_004429.1
Ncbi Gene Id Number:
2043
Background:
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
Background Reference 1:
Fu WY, et al. 2007. Nat Neurosci. 10:67-76.
Background Reference 2:
Miao H, et al. 2009. Cancer Cell. 16(1):9-20.
Background Reference 3:
Zhang G, et al. 2008. Cancer Res. 68(6):1691-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-257

List Size:
0.1 mg

List Price:

$515.00

Datasheet
Shipping Info

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