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45-544 (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-544 (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EPHB2 Antibody

CATALOG NUMBER: 45-544

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-QKDRNHRPKFGQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60kDa band observed in Human Lung and Placenta lysates (calculated MW of 110kDa according to NP_059145.2 and NP_004433.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Litterst et al, J Biol Chem. 2007 Apr 10; PMID: 17428795). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms ( NP_059145.2 and NP_004433.2).
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EPHB2
Additional Names:
EPHB2, DRT, EPH receptor B2, Cek5, Qek5, Sek3, ETECK, Prkm5, EPHT3, ERK, Hek5, MGC87492, Tyro5, developmentally-regulated eph-related tyrosine kinase, elk-related tyrosine kinase, eph tyrosine kinase 3, ephrin receptor EphB2
Protein Accession Number:
NP_004433.2,NP_059145.2
Protein Gi Number:
21396504
Ncbi Gene Id Number:
2048
Background Reference 1:
Cramer KS, Cerretti DP, Siddiqui SA. EphB2 regulates axonal growth at the midline in the developing auditory brainstem. Dev Biol. 2006 Apr 13;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-544

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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