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Western blot analysis of anti-EphB6 N-term Pab in A549 cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Flow cytometric analysis of MCF-7 cells using EphB6 Antibody (N-term S45) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti-EphB6 N-term Pab in A549 cell lysate

EphB6 Antibody

CATALOG NUMBER: 63-064

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This EphB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human EphB6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
111 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EPHB6
Additional Names:
Ephrin type-B receptor 6, HEP, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6
Protein Accession Number:
O15197
Ncbi Gene Id Number:
2051
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.
Background Reference 1:
Freywald, A., et al., J. Biol. Chem. 278(12):10150-10156 (2003).
Background Reference 2:
Luo, H., et al., J. Clin. Invest. 110(8):1141-1150 (2002).
Background Reference 3:
Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001).
Background Reference 4:
Luo, H., et al., J. Immunol. 167(3):1362-1370 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-064

List Size:
400 ul

List Price:

$495.00

Datasheet
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