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The purity of rh Ephrin-A1 /EFNA1 Fc Chimera was determined by DTT-reduced (+) and non-reduced (-) SDS-PAGE and staining overnight with Coomassie Blue.
The purity of rh Ephrin-A1 /EFNA1 Fc Chimera was determined by DTT-reduced (+) and non-reduced (-) SDS-PAGE and staining overnight with Coomassie Blue.

Ephrin A1 Recombinant Protein

CATALOG NUMBER: 96-256

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Asp 19 - Ser 182
Fusion Tag:
C-Fc Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
46 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. When Recombinant Human EphA2 is coated at 4 μg/mL (100 μl/well), the concentration of rhEphrin-A1 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0. 5 - 4 ng/mL.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
50 mM tris, 100 mM glycine, pH7.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
EFNA1
Additional Names:
EFNA1, Ephrin-A1, LERK-1, TNFAIP4, EPLG1, B61, ECKLG, EFL1
Protein Accession Number:
NP_004419
Ncbi Gene Id Number:
1942
Background:
Ephrin-A1 (EFNA1) is also known as EPLG1, LERK1, TNFAIP4, Immediate early response protein B61, which belongs to the ephrin family. EFNA1 contains one ephrin RBD (ephrin receptor-binding) domain. EFNA1 is cell surface GPI-bound ligand for Eph receptors, and is a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. EFNA1 binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. EFNA1 plays an important role in angiogenesis and tumor neovascularization.
Background Reference 1:
Liu D.-P., et al., 2007, Int. J. Oncol. 30:865-871.
Background Reference 2:
Wykosky J., et al., 2008, Oncogene 27:7260-7273.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-256

List Size:
0.1 mg

List Price:

$463.00

Datasheet
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