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The purity of rh Ephrin-B1 /EFNB1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
The purity of rh Ephrin-B1 /EFNB1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.

Ephrin B1 Recombinant Protein

CATALOG NUMBER: 96-255

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Leu 28 - Gly 232
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
23.2 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant human EphrinB1 at 10μg/ml (100μl/well) can bind human EphB6 with a linear range of 0. 2-8μg/ml.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
EFNB1
Additional Names:
EFNB1, EFL-3, ELK ligand, ELK-L, EPLG2, LERK2, CFND, CFNS
Protein Accession Number:
NP_004420
Ncbi Gene Id Number:
1947
Background:
Ephrin-B1 (EFNB1) is also known as EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2 (LERK-2), which belongs to the ephrin family. EFNB1 contains one ephrin RBD (ephrin receptor-binding) domain. EFNB1 binds to the receptor tyrosine kinases EPHB1 and EPHA1. EFNB1 binds to, and induce the collapse of, commissural axons/growth cones in vitro. EFNB1 may play a role in constraining the orientation of longitudinally projecting axons. EFNB1 interacts with GRIP1 and GRIP2.
Background Reference 1:
Zhang Z., et al., 2004, Protein Sci. 13:2819-2824.
Background Reference 2:
Brueckner K., et al., 1999, Neuron 22:511-524.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-255

List Size:
0.2 mg

List Price:

$463.00

Datasheet
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