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Western blot analysis of lysed extracts from HT29 cells using Ephrin-B2 (Ab-330).
Immunofluorescence staining of methanol-fixed HeLa cells using Ephrin-B2 (Ab-330).
Western blot analysis of lysed extracts from HT29 cells using Ephrin-B2 (Ab-330).

Ephrin B2 (Ab 330) Antibody

CATALOG NUMBER: 79-576

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Ephrin-B2 (Ab-330) antibody was raised against a peptide sequence around aa.328~332 (N-I-Y-Y-K) derived from Human Ephrin B (ephrin-B2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total Ephrin-B2 protein.
Predicted Molecular Weight:
37 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EFNB2
Additional Names:
HTKL, EPLG5, Htk-L, LERK5, ON, EFNB2, HTKL, LERK5
Protein Accession Number:
NP_004084.1
Protein Gi Number:
4758250
Ncbi Gene Id Number:
1948
Background:
Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.
Background Reference 1:
Chrencik JE, et al. ( 2006) J Biol Chem;281 (38): 28185-28192. Kertesz N, et al. (2006) Blood; 107 (6): 2330-2338. Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101 (15): 5583-5588.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-576

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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