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Western blot analysis of lysed extracts from HT29 cells, untreated or treated with UV using Ephrin-B2 (Phospho-Tyr330).
Western blot analysis of lysed extracts from HT29 cells, untreated or treated with UV using Ephrin-B2 (Phospho-Tyr330).

Ephrin B2 (phospho Tyr330) Antibody

CATALOG NUMBER: 79-466

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Ephrin-B2 (Phospho-Tyr330) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 330 (N-I-Y (p) -Y-K) derived from Human Ephrin-B2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 330.
Predicted Molecular Weight:
37 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EFNB2
Additional Names:
HTKL, EPLG5, Htk-L, LERK5, NR1C3, HTKL, MGC126226
Protein Accession Number:
NP_004084.1
Protein Gi Number:
4758250
Ncbi Gene Id Number:
1948
Background:
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Background Reference 1:
Chrencik JE, et al. ( 2006) J Biol Chem;281 (38): 28185-28192. Kertesz N, et al. (2006) Blood; 107 (6): 2330-2338. Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101 (15): 5583-5588.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-466

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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