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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis in Hela cell line lysates (35ug/lane).

EPN1 Antibody

CATALOG NUMBER: 58-346

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This EPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-232 amino acids from the Central region of human EPN1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EPN1
Additional Names:
Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1
Protein Accession Number:
Q9Y6I3
Protein Gi Number:
332278179
Ncbi Gene Id Number:
29924
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EPN1 is an endocytic accessory protein that interacts with EPS15 (MIM 600051), the alpha subunit of the clathrin adaptor AP2 (AP2A1; MIM 601026), and clathrin (see MIM 118960), as well as with other accessory proteins for the endocytosis of clathrin-coated vesicles.
Background Reference 1:
Liu, Z., et al. J. Cell Biol. 186(4):473-480(2009) Kazazic, M., et al. Traffic 10(2):235-245(2009) Olsen, J.V., et al. Cell 127(3):635-648(2006) Olsen, J.V., et al. Cell 127(3):635-648(2006) Schmid, E.M., et al. PLoS Biol.(9), E262 (2006) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-346

List Size:
400 ul

List Price:

$495.00

Datasheet
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