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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of EPOR Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

EPOR Antibody

CATALOG NUMBER: 58-645

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EPOR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-357 amino acids from the Central region of human EPOR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EPOR
Additional Names:
Erythropoietin receptor, EPO-R, EPOR
Protein Accession Number:
P19235
Protein Gi Number:
119524
Ncbi Gene Id Number:
2057
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the erythropoietin receptor which is amember of the cytokine receptor family. Upon erythropoietinbinding, this receptor activates Jak2 tyrosine kinase whichactivates different intracellular pathways including: Ras/MAPkinase, phosphatidylinositol 3-kinase and STAT transcriptionfactors. The stimulated erythropoietin receptor appears to have arole in erythroid cell survival. Defects in the erythropoietinreceptor may produce erythroleukemia and familial erythrocytosis.Dysregulation of this gene may affect the growth of certain tumors.Alternate splicing results in multiple transcript variants.
Background Reference 1:
Lim, A.C., et al. Biochemistry 49(18):3797-3804(2010)
Background Reference 2:
Perrotta, S., et al. PLoS ONE(8), E12015 (2010) :
Background Reference 3:
Khankin, E.V., et al. PLoS ONE(2), E9246 (2010) :
Background Reference 4:
Wincewicz, A., et al. Folia Histochem. Cytobiol. 47(3):425-430(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-645

List Size:
400 ul

List Price:

$495.00

Datasheet
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