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Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

EPRS Antibody

CATALOG NUMBER: 62-995

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EPRS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-314 amino acids from the N-terminal region of human EPRS.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:8000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
171 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EPRS
Additional Names:
Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase, Cell proliferation-inducing gene 32 protein, Glutamatyl-prolyl-tRNA synthetase, Glutamate--tRNA ligase, Glutamyl-tRNA synthetase, GluRS, Proline--tRNA ligase, Prolyl-tRNA synthetase, EPRS, GLNS, PARS, QARS, QPRS
Protein Accession Number:
P07814
Protein Gi Number:
288558855
Ncbi Gene Id Number:
2058
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. EPRS is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species.
Background Reference 1:
Jia,J., Mol. Cell 29 (6), 679-690 (2008)
Background Reference 2:
Beausoleil,S.A., Nat. Biotechnol. 24 (10), 1285-1292 (2006)
Background Reference 3:
Kato,T., Cancer Res. 65 (13), 5638-5646 (2005)
Background Reference 4:
Sampath,P., Cell 119 (2), 195-208 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-995

List Size:
400 ul

List Price:

$495.00

Datasheet
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