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45-548 (5ug/ml) staining of paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-548 (5ug/ml) staining of paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

EPS8 Antibody

CATALOG NUMBER: 45-548

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-SGVESFDEGSSH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Breast Carcinoma shows staining of cancer cells infiltrated in a breast lobe. Recommended concentration, 5-10ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EPS8
Additional Names:
EPS8, epidermal growth factor receptor pathway substrate 8
Protein Accession Number:
NP_004438.3
Protein Gi Number:
56682953
Ncbi Gene Id Number:
2059
Background Reference 1:
Fazioli F, Minichiello L, Matoska V, Castagnino P, Miki T, Wong WT, Di Fiore PP. Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals. EMBO J. 1993 Oct;12(10):3799-808.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-548

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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