Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

IHC testing of human breast invasive ductal carcinoma with ER antibody (ER506).
Intracellular FACS staining of MCF-7 cells using ER antibody (red) and isotype control (green).
FACS testing of human MCF-7: Black=cells alone; Green=isotype control; Red= Estrogen Receptor PE conjugate
Western blot testing of MCF-7 cell lysate with ER antibody (clone ER506). Predicted molecular weight of ER alpha: ~66kDa.
IHC testing of human breast invasive ductal carcinoma with ER antibody (ER506).

ER Antibody [ER506]

CATALOG NUMBER: 33-127

Clonality:
Monoclonal
Tested Applications:
Flow, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, WB
Application Note:
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IHC (Formalin-paraffin): 1-2 ug/ml (1)
Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.

1. FFPE testing requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min and cooling at RT for 20 minutes.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
ESR1
Additional Names:
ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER
Ncbi Gene Id Number:
2099
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Clone ER506 antibody strongly stains nuclei of epithelial cells in breast carcinomas.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-127

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369