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Fluorescent confocal image of SY5Y cells stained with ERAS (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ERAS (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.
Western blot analysis of ERAS Antibody (F66) in HL60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (F66) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Fluorescent confocal image of SY5Y cells stained with ERAS (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ERAS (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.

ERAS Antibody

CATALOG NUMBER: 57-928

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-81 amino acids from the N-terminal region of human ERAS.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IF starting dilution is: 1:100
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
25 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ERAS
Additional Names:
GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, ERAS, HRAS2, HRASP
Protein Accession Number:
Q7Z444
Protein Gi Number:
74723320
Ncbi Gene Id Number:
3266
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.
Background Reference 1:
References for protein:
Background Reference 2:
Kameda,T., Stem Cells 23 (10), 1535-1540 (2005)
Background Reference 3:
Takahashi,K., Nature 423 (6939), 541-545 (2003)
Background Reference 4:
Miyoshi,J., Nucleic Acids Res. 12 (4), 1821-1828 (1984)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-928

List Size:
400 ul

List Price:

$495.00

Datasheet
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