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Overlay histogram showing HepG2 cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antibody staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: A549 whole cell lysates Lane 3: NCI-H1299 whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysates Lane 2: human placenta lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
Overlay histogram showing HepG2 cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

EREG Antibody

CATALOG NUMBER: 61-668

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EREG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human EREG.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For FACS starting dilution is: 1:25
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EREG
Additional Names:
Proepiregulin, Epiregulin, EPR, EREG
Protein Accession Number:
O14944
Ncbi Gene Id Number:
2069
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EREG is a member of the epidermal growth factor family. EREG can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors.
Background Reference 1:
Ben-Ami, I., et al. Hum. Reprod. 24(1):176-184(2009)
Background Reference 2:
Cho, M.C., et al. Biochem. Biophys. Res. Commun. 377(3):832-837(2008)
Background Reference 3:
Lasky-Su, J., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-668

List Size:
400 ul

List Price:

$495.00

Datasheet
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