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Western blot analysis in 293 cell line lysates (35ug/lane).
ERG25 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tisssue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in 293 cell line lysates (35ug/lane).

ERG25 Antibody

CATALOG NUMBER: 57-377

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ERG25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human ERG25.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MSMO1
Additional Names:
Methylsterol monooxygenase 1, C-4 methylsterol oxidase, MSMO1, DESP4, ERG25, SC4MOL
Protein Accession Number:
Q15800
Protein Gi Number:
2498340
Ncbi Gene Id Number:
6307
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Sterol-C4-mehtyl oxidase-like protein was isolated basedon its similarity to the yeast ERG25 protein. It contains a set ofputative metal binding motifs with similarity to that seen in afamily of membrane desaturases-hydroxylases. The protein islocalized to the endoplasmic reticulum membrane and is believed tofunction in cholesterol biosynthesis. Alternatively splicedtranscript variants encoding distinct isoforms have been found forthis gene.
Background Reference 1:
Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008)
Background Reference 2:
Lim, J., et al. Cell 125(4):801-814(2006)
Background Reference 3:
Li, L., et al. J. Biol. Chem. 271(28):16927-16933(1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-377

List Size:
400 ul

List Price:

$495.00

Datasheet
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