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Western blot of human T47D cells showing phospho-specific immunolabeling of the ~42k-44k ERK/MAPK protein doublet.
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~42k-44k ERK/MAPK protein doublet.

ERK1 / 2 (phospho Thr202 / Tyr204) Antibody

CATALOG NUMBER: XPS-1009

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Immunogen:
ERK/MAPK (Thr202/Tyr204) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. Dot blots and Western blots with a rat brain lysate were performed with each lot. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
ERK1/2 antibody is specific for the 42/44 k ERK/MAPK protein phosphorylated at Thr202 and Tyr204..
Predicted Molecular Weight:
42/44
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
Ncbi Official Symbol:
Mapk1
Protein Accession Number:
P63086
Protein Gi Number:
52001075
Ncbi Gene Id Number:
116590
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.
Background Reference 1:
Tanoue, T.J. and Nishida, E., "Molecular recognitions in the MAP kinase cascades. Cellular Signaling 15 (2003) 455 - 462.
Background Reference 2:
Adams, J.P. and Sweatt, J.D., "Molecular psychology: Roles for the ERK MAP kinase cascade in memory," Annu. Rev. Pharmacol. Toxicol., 42 (2002) 135 - 163.
Background Reference 3:
Johnson, G.L. and Lapadat, R., "Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases," Science, 298 (2002) 1911 - 1912.
Background Reference 4:
Atkins, C.M., Selcher, J.C., Petraitis, J.J., Trzaskos, J.M., and Sweatt, J.D., "The MAPK cascade is required for mammalian associative learning," Nat. Neurosci., 1 (1998) 602-609.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1009

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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