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Western Blot at 1:500 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:500 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.

ERK3 Antibody

CATALOG NUMBER: 62-953

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ERK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 692~721 amino acids from the C-terminal region of human ERK3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
For WB starting dilution is: 1:500
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPK6
Additional Names:
Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6, Extracellular signal-regulated kinase 3, ERK-3, MAP kinase isoform p97, p97-MAPK, MAPK6, ERK3, PRKM6
Protein Accession Number:
Q16659
Protein Gi Number:
2499596
Ncbi Gene Id Number:
5597
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Background Reference 1:
Coulombe, P., et al., Mol. Cell. Biol. 23(13):4542-4558 (2003).
Background Reference 2:
Meloche, S., et al., Oncogene 13(7):1575-1579 (1996).
Background Reference 3:
Cheng, M., et al., J. Biol. Chem. 271(15):8951-8958 (1996).
Background Reference 4:
Zhu, A.X., et al., Mol. Cell. Biol. 14(12):8202-8211 (1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-953

List Size:
400 ul

List Price:

$495.00

Datasheet
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