Western blot analysis of extracts of various cell lines, using ERLIN1 antibody (15-759) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human appendix using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ERLIN1 antibody (15-759) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ERLIN1 Antibody

CATALOG NUMBER: 15-759

Clonality:

Polyclonal

Tested Applications:

IHC, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2).

Conjugate:

Unconjugated

Tested Applications:

IHC, WB

Application Note:

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Positive Control 1:

293T

Positive Control 2:

HepG2

Positive Control 3:

BxPC-3

Positive Control 4:

DU145

Positive Control 5:

OVCAR3

Positive Control 6:

Mouse kidney

Predicted Molecular Weight:

Observed: 39kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

ERLIN1

Additional Names:

ERLIN1, C10orf69, Erlin-1, KE04, KEO4, RP11-316M21.1, SPFH1, SPG62

Ncbi Gene Id Number:

10613

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

The protein encoded by this gene is part of a protein complex that mediates degradation of inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum. The encoded protein also binds cholesterol and regulates the SREBP signaling pathway, which promotes cellular cholesterol homeostasis. Defects in this gene have been associated with spastic paraplegia 62.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-759

List Size:

100 uL

List Price:

$436.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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ERLIN1 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Positive Control 3:

Positive Control 4:

Positive Control 5:

Positive Control 6:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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