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45-552 staining (1ug/ml) of Human Breast lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
45-552 (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-552 staining (1ug/ml) of Human Breast lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

ERLIN2 Antibody

CATALOG NUMBER: 45-552

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-EDEPLETATKEN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 43kDa band observed in Human Breast lysates and in lysates of Human Breast Cancer (calculated MW of 37.8kDa according to NP_009106.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pearce et al, J Biol Chem. 2007 Jul 13;282(28):20104-15.; PMID: 17502376). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognise isoform 1 (NP_009106) only.
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ERLIN2
Additional Names:
ERLIN2, SPFH2, ER lipid raft associated 2, SPFH domain family, member 2 , HGNC:1356, C8orf2, MGC87072 , chromosome 8 open reading frame 2, Erlin-2, MGC87072
Protein Accession Number:
NP_009106.1
Protein Gi Number:
6005721
Ncbi Gene Id Number:
11160
Background Reference 1:
Garcia MJ, Pole JC, Chin SF, Teschendorff A, Naderi A, Ozdag H, Vias M, Kranjac T, Subkhankulova T, Paish C, Ellis I, Brenton JD, Edwards PA, Caldas C. A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes. Oncogene. 2005 Aug 4;24(33):5235-45.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-552

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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