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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).  
ERO1LB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).  

ERO1LB Antibody

CATALOG NUMBER: 61-837

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ERO1LB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-293 amino acids from the C-terminal region of human ERO1LB.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ERO1LB
Additional Names:
ERO1-like protein beta, ERO1-L-beta, 184-, Endoplasmic reticulum oxidoreductin-1-like protein B, Oxidoreductin-1-L-beta, ERO1LB
Protein Accession Number:
Q86YB8
Protein Gi Number:
116241353
Ncbi Gene Id Number:
56605
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ERO1LB is essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1LB acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. ERO1LB does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent.
Background Reference 1:
Landa, I., et al. PLoS Genet.(9), E1000637 (2009) Savu, M.K., et al. Surg Obes Relat Dis 5(2):172-180(2009) Tavendale, R., et al. J. Allergy Clin. Immunol. 121(4):860-863(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-837

List Size:
400 ul

List Price:

$495.00

Datasheet
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