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46-744 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-744 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ERP29 Antibody

CATALOG NUMBER: 46-744

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EKNKMSDGKKEELQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 28kDa band observed in Human Liver lysates (calculated MW of 29.0kDa according to NP_006808.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. This product has been successfully used in WB on Rat: Gao J et al, Mol Med Rep. 2016 Jan;13(1):398-402. PMID: 26548430.Immunofluorescence:This product has been successfully used in IF on Rat: Gao J et al, Mol Med Rep. 2016 Jan;13(1):398-402. PMID: 26548430.
Specificity:
This antibody is expected to recognize isoform 1 (NP_006808.1) only.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ERP29
Additional Names:
ERP29, endoplasmic reticulum protein 29, C12orf8, ERp28, ERp31, PDI-DB, endoplasmic reticulum lumenal protein ERp28
Protein Accession Number:
NP_006808.1
Protein Gi Number:
5803013
Ncbi Gene Id Number:
10961
Background Reference 1:
Rainey-Barger EK, Magnuson B, Tsai B. A chaperone-activated nonenveloped virus perforates the physiologically relevant endoplasmic reticulum membrane. J Virol. 2007 Dec;81(23):12996-3004.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-744

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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