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Western blot analysis of ERRF in (A) HeLa and (B) A-20 cell lysate with ERRF antibody at 1 μg/mL.
Immunocytochemistry of ERRF in HeLa cells with ERRF antibody at 2.5 μg/ml.
Immunofluorescence of ERRF in HeLa cells with ERRF antibody at 20 μg/ml.
Western blot analysis of ERRF in (A) HeLa and (B) A-20 cell lysate with ERRF antibody at 1 μg/mL.
Trial Size

ERRF Antibody

CATALOG NUMBER: 7289

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ERRF antibody was raised against a 15 amino acid peptide near the carboxy terminus of human ERRF .
The immunogen is located within the last 50 amino acids of ERRF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ERRF Antibody can be used for detection of ERRF by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 3:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 19 kDa
Observed: 15 kDa
Purification:
ERRF Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ERRF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ERRF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
C1orf64
Additional Names:
ERRF Antibody: ERRF, Uncharacterized protein C1orf64
Protein Accession Number:
NP_849162
Protein Gi Number:
30519894
Ncbi Gene Id Number:
149563
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ERRF Antibody: The estrogen receptor-related factor (ERRF) is a recently identified protein whose expression is upregulated in multiple cell lines and primary breast cancer tumors. Increased ERRF expression was significantly associated with ER or progesterone receptor (PR) positivity even in HER2-negative tumors. This higher ERRF expression correlated with better disease-free survival and overall survival. In two ER-positive breast cancer cell lines, knockdown of ERRF suppressed cell growth in vitro and tumorigenesis in xenograft models, suggesting that ERRF plays a role in estrogen-ER-mediated breast cancer cell growth and may thus be a potential therapeutic target.
Background Reference 1:
Su D, Fu X, Fan S, et al. Role of ERRF, a novel ER-related nuclear factor, in the growth control of ER-positive human breast cancer cells. Am. J. Pathol. 2012; 180:1189-201.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7289

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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