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42-697 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-697 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ERV3 Antibody

CATALOG NUMBER: 42-697

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PQDNFTLTASS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.9kDa according to NP_001007254.2. The 30kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml. This product has been successfully used in IHC on Human: Frese S et al, PLoS One. 2015 Jul 8;10(7). PMID: 26154387.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ERV3
Additional Names:
ERV3, endogenous retroviral sequence 3 (includes zinc finger protein H-plk/HPF9), ERV-R, H-PLK, HERV-R, ERV-3 envelope protein, endogenous retroviral sequence 3, envelope polyprotein
Protein Accession Number:
NP_001007254.2
Protein Gi Number:
145651814
Ncbi Gene Id Number:
2086

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-697

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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