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Western Blot at 1:2000 dilution + Rat kidney lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + Recombinant human EPO protein Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: H. kidney whole cell lysate Lane 2: H. liver whole cell lysate Lane 3: M. liver whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + Rat kidney lysate Lysates/proteins at 20 ug per lane.

Erythropoietin Antibody

CATALOG NUMBER: 58-412

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This Erythropoietin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-48 amino acids from the N-terminal region of human Erythropoietin.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EPO
Additional Names:
Erythropoietin, Epoetin, EPO
Protein Accession Number:
P01588
Protein Gi Number:
119526
Ncbi Gene Id Number:
2056
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EPO is a member of the EPO/TPO family and encodes asecreted, glycosylated cytokine composed of four alpha helicalbundles. The protein is found in the plasma and regulates red cellproduction by promoting erythroid differentiation and initiatinghemoglobin synthesis. This protein also has neuroprotectiveactivity against a variety of potential brain injuries andantiapoptotic functions in several tissue types. [provided byRefSeq].
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Kristensen, P.L., et al. Eur. J. Endocrinol. 163(3):391-398(2010)
Background Reference 3:
Kim, M.H., et al. Mol. Biol. (Mosk.) 44(4):656-663(2010)
Background Reference 4:
Carraway, M.S., et al. Circ. Res. 106(11):1722-1730(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-412

List Size:
400 ul

List Price:

$495.00

Datasheet
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