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Western blot analysis of ESR1 in rat brain tissue lysate with ESR1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of ESR1 in rat brain tissue with ESR1 antibody at 5 μg/mL.
Immunofluorescence of ESR1 in rat brain tissue with ESR1 antibody at 20 μg/mL.
Western blot analysis of ESR1 in rat brain tissue lysate with ESR1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ESR1 Antibody

CATALOG NUMBER: 7437

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Horse: (100%)
Immunogen:
ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1.
The immunogen is located within amino acids 250 - 300 of ESR1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
ESR1 antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 65 kDa
Observed: 62 kDa
Purification:
ESR1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ESR1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ESR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ESR1
Additional Names:
ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER
Protein Accession Number:
NP_001116214
Protein Gi Number:
170295804
Ncbi Gene Id Number:
2099
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ESR1 Antibody: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).
Background Reference 1:
Pakdel F, Reese JC, and Katzenellenbogen BS. Identification of charged residues in an N- terminal portion of the hormone-binding domain of the human estrogen receptor important in transcriptional activity of the receptor. Mol. Endocrinol. 1993; 7:1408-17.
Background Reference 2:
Sheeler CQ, Singleton DW, and Khan SA. Mutation of serines 104, 106, and 118 inhibits dimerization of the human estrogen receptor in yeast. Endocr. Res. 2003; 29:237-55.
Background Reference 3:
Rogatsky I, Trowbridge JM, and Garabedian MJ. Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex. J. Biol. Chem. 1999; 274:22296-302.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7437

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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