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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

ESR2 Antibody

CATALOG NUMBER: 27-386

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ESR2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ESR2 antibody can be used for detection of ESR2 by ELISA at 1:312500. ESR2 antibody can be used for detection of ESR2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
48 kDa, 55 kDa, 55 kDa, 59 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ESR2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ESR2
Additional Names:
ESR2, Erb, ESRB, ESTRB, NR3A2, ER-BETA, ESR-BETA
Protein Accession Number:
Q92731
Protein Gi Number:
6166154
Ncbi Gene Id Number:
2100
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ESR2 is a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.
Background Reference 1:
Brandenberger, A.W., et al., (1997) J. Clin. Endocrinol. Metab. 82 (10), 3509-3512.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-386

List Size:
100 ul

List Price:

$473.00

Datasheet
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