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46-655 (0.2ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing ESRRG and probed with 46-655 (mock transfection in first lane).
46-655 (0.2ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ESRRG Antibody

CATALOG NUMBER: 46-655

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: CRMSNKDRHIDS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 50kDa band observed in Human Brain (Cerebellum) and Breast lysates (calculated MW of 51.3kDa according to NP_NP_001429.2). In transfected HEK293 transiently expressing ESRRG a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6ug/ml.
Specificity:
This antibody is expected to recognise the reported isoform NP_001429.2.
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ESRRG
Additional Names:
ESRRG, DKFZp781L1617, Estrogen-related receptor gamma, ERR3, FLJ16023, KIAA0832, NR3B3
Protein Accession Number:
NP_001429.2,NP_996317.1,NP_996318.1
Protein Gi Number:
45545433
Ncbi Gene Id Number:
2104
Background Reference 1:
Dufour CR, Wilson BJ, Huss JM, Kelly DP, Alaynick WA, Downes M, Evans RM, Blanchette M, Giguere V. Genome-wide orchestration of cardiac functions by the orphan nuclear receptors ERRalpha and gamma. Metab. 2007 May;5(5):345-56.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-655

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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