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Western blot analysis of lysed extracts from MCF cells using Estrogen Receptor-α (Ab-167).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-167).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-α (Ab-167).
Western blot analysis of lysed extracts from MCF cells using Estrogen Receptor-α (Ab-167).

Estrogen Receptor a (Ab 167) Antibody

CATALOG NUMBER: 79-306

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Estrogen Receptor-a (Ab-167) antibody was raised against a peptide sequence around aa.165~169 (L-A-S-T-N) derived from Human Estrogen Receptor-α.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total Estrogen Receptor-α protein.
Predicted Molecular Weight:
66 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
ESR1
Additional Names:
ER, ESR, Era, ESRA, ESTRR, NR3A1, LIMK, ER, ESR1, ESTR, ESTRA
Protein Accession Number:
NP_000116.2
Protein Gi Number:
62821794
Ncbi Gene Id Number:
2099
Background:
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Background Reference 1:
H Yamaguchi, J Zhu, T Yu, et al. (2007) Serum-free mouse embryo cells generate a self-sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their differentiation state. Cell Biology International, 31 (6): 638-644.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-306

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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