Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of ESX1 in human testis tissue lysate with ESX1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of ESX1 in human testis tissue with ESX1 antibody at 2.5 μg/mL.
Immunofluorescence of ESX1 in human testis tissue with ESX1 antibody at 20 μg/mL.
Western blot analysis of ESX1 in human testis tissue lysate with ESX1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ESX1 Antibody

CATALOG NUMBER: 5597

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (71%), Mouse: (71%)
Immunogen:
ESX1 antibody was raised against a 19 amino acid synthetic peptide near the center of human ESX1.
The immunogen is located within amino acids 140 - 190 of ESX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ESX1 antibody can be used for detection of ESX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Purification:
ESX1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ESX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ESX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ESX1
Additional Names:
ESX1 Antibody: ESX1L, ESXR1, ESX1L, ESX1R, Homeobox protein ESX1, Extraembryonic, spermatogenesis, homeobox 1
Protein Accession Number:
Q8N693
Protein Gi Number:
116241356
Ncbi Gene Id Number:
80712
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ESX1 Antibody: Homeobox proteins are transcription factors that contain a helix-turn-helix DNA binding domain termed the homeodomain. ESX1 is an X-linked homeobox gene primarily expressed in the placenta and testis and contains two functional domains: the homeodomain and the proline-rich domain. During embryogenesis, ESX1 is expressed in the extraembryonic tissues, including the endoderm of the visceral yolk sac, the ectoderm of the chorion and the labyrinthine trophoblast of the chorioallantoic placenta. ESX1 can act like a transcriptional repressor to the human oncogene K-ras and treatment of human cancer cells with an ESX1 protein fragment containing the homeodomain reduces the tumorgenicity of cells containing oncogenic K-ras mutations, suggesting ESX1 may be useful as a therapeutic treatment for these cancers.
Background Reference 1:
Gehring WJ, Affolter M and Burglin T. Homeodomain proteins. Annu. Rev. Biochem.1994; 63:487-526.
Background Reference 2:
Fohn LE and Behringer RR. ESX1L, a novel X chromosome-linked human homeobox gene expressed in the placenta and testis. Genomics2001; 74:105-8.
Background Reference 3:
Li Y, Lemaire P and Behringer RR. ESX1, a novel X chromosome-linked homeobox gene expressed in mouse extraembryonic tissues and male germ cells. Dev. Biol.1997; 188:85-95.
Background Reference 4:
Figueiredo AL, Salles MG, Albano RM, et al. Molecular and morphogenic analyses of expression of ESX1L in different stages of human placental development. J. Cell Mol. Med.2004; 8:545-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5597

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369