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Western blot analysis of extracts of various cell lines, using ETF1 antibody (19-944) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunofluorescence analysis of NIH-3T3 cells using ETF1 Polyclonal Antibody (19-944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ETF1 antibody (19-944) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

ETF1 Antibody

CATALOG NUMBER: 19-944

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 278-437 of human ETF1 (NP_004721.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:200 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
Raji
Positive Control 2:
U-87MG
Positive Control 3:
HT-1080
Positive Control 4:
SKOV3
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 55kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ETF1
Additional Names:
D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1, eukaryotic peptide chain release factor subunit 1, polypeptide chain release factor 1, protein Cl1, sup45 (yeast omnipotent suppressor 45) homolog-like 1
Ncbi Gene Id Number:
2107
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-944

List Size:
100 uL

List Price:

$436.00

Datasheet
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