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42-619 (0.1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-619 (5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-619 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-619 (0.1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ETFA Antibody

CATALOG NUMBER: 42-619

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KSPDTFVRTIYAGN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 30+35kDa bands observed in Human Colon lysates (calculated MW of 35.1kDa according to NP_000117.1 and of 30.0kDa according to NP_001121188.1. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland and Heart. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both isoforms (NP_000117.1; NP_001121188.1). Amino acid numbering in name refers to NP_000117.1 sequence)
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Positive Control 2:
Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
Positive Control 3:
Cat. No. 1468 - Rat Small Intestine Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ETFA
Additional Names:
ETFA, electron-transfer-flavoprotein, alpha polypeptide, EMA, GA2, MADD, electron transfer flavoprotein alpha-subunit, electron transfer flavoprotein, alpha polypeptide, glutaric aciduria II
Protein Accession Number:
NP_000117.1,NP_001121188.1
Protein Gi Number:
4503607
Ncbi Gene Id Number:
2108

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-619

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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