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42-808 (0.01ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-808 (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-808 (0.01ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ETFB Antibody

CATALOG NUMBER: 42-808

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-PQRTAGVKVETTE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 27.8kDa according to NP_001976.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Positive Control 3:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ETFB
Additional Names:
ETFB, electron-transfer-flavoprotein, beta polypeptide, FP585, MADD, electron transfer flavoprotein beta subunit, electron transfer flavoprotein beta-subunit, electron transfer flavoprotein, beta polypeptide, electron-transferring-flavoprotein, beta polypeptide
Protein Accession Number:
NP_001976.1,NP_001014763.1
Protein Gi Number:
4503609
Ncbi Gene Id Number:
2109

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-808

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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