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Antibody used in WB on human LCL, mouse brains at 1:1000.
Antibody used in WB on Human kidney at 0.2-1 ug/ml.
Antibody used in WB on human LCL, mouse brains at 1:1000.

ETS2 Antibody

CATALOG NUMBER: 31-278

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Drosophila, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Drosophila, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ETS2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ETS2 antibody can be used for detection of ETS2 by ELISA at 1:312500. ETS2 antibody can be used for detection of ETS2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10408 - Fetal Kidney Tissue Lysate
Predicted Molecular Weight:
53 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ETS2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ETS2
Additional Names:
ETS2, ETS2IT1
Protein Accession Number:
NP_005230
Protein Gi Number:
4885221
Ncbi Gene Id Number:
2114
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ETS2 contains an ETS DNA-binding domain and belongs to the ETS family. ETS2 is a target of protein kinase C and upregulates GM-CSF. Ets2 and its targets play essential roles in endothelial cell function. Coexpression of Ets-2 and SRC-1 significantly associated with the rate of recurrence and HER expression in breast cancer. Overexpression of ETS2 is associated with human esophageal squamous cell carcinoma. ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).
Background Reference 1:
Ge, Y., (2008) Leukemia 22 (3), 521-529 .

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-278

List Size:
100 ul

List Price:

$519.00

Datasheet
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