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Western blot analysis of ETS2 Antibody in MCF-7 cell line lysates (35ug/lane)
ETS2 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ETS2 Antibody in MCF-7 cell line lysates (35ug/lane)

ETS2 Antibody

CATALOG NUMBER: 64-143

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ETS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-173 amino acids from the Central region of human ETS2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ETS2
Additional Names:
Protein C-ets-2, ETS2
Protein Accession Number:
P15036
Protein Gi Number:
119645
Ncbi Gene Id Number:
2114
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes.
Background Reference 1:
Zabuawala, T., et al. Cancer Res. 70(4):1323-1333(2010)
Background Reference 2:
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Background Reference 3:
Shalaby, A.A., et al. Mod. Pathol. 22(8):996-1005(2009)
Background Reference 4:
Sutter, W., et al.Craniomaxillofac Surg 37(5):263-271(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-143

List Size:
400 ul

List Price:

$495.00

Datasheet
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