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Western blot analysis of ETV4 in (A) human brain tissue lysate and (B) A431 cell lysate with ETV4 antibody at 1 μg/ml.
Western blot analysis of ETV4 in (A) human brain tissue lysate and (B) A431 cell lysate with ETV4 antibody at 1 μg/ml.
Trial Size

ETV4 Antibody

CATALOG NUMBER: 8535

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish: (72%)
Immunogen:
ETV4 antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human ETV4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ETV4 antibody can be used for Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of ETV4 are known to exist. This antibody will only detect the two longest isoforms.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 1202 - A431 Cell Lysate
Predicted Molecular Weight:
Predicted: 53 kDa
Observed: 53 kDa
Purification:
ETV4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ETV4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ETV4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ETV4
Additional Names:
ETV4 Antibody: ETS variant 4, E1AF, PEA3, E1A-F, PEAS3
Protein Accession Number:
NP_001977
Protein Gi Number:
24307883
Ncbi Gene Id Number:
2118
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ETV4 Antibody: ETV4, along with the related proteins ETV1 and ETV5, form the PEA3 subfamily of the E26 transformation-specific (ETS) transcription factor family (1). ETV4 is thought to play roles in embryonic kidney development and fertility (2,3), and is also thought to be an oncoprotein in multiple cancers (4).
Background Reference 1:
Xin JH, Cowie A, LAchance P, et al. Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells. Genes Dev. 1992; 6:481-96.
Background Reference 2:
Lu BC, Cebrian C, Chi X, et al. Etv4 and Etv5 are required downstream of GDNF and Ret for kidney branching morphogenesis. Nat. Genet. 2009; 41:1295-302.
Background Reference 3:
Laing MA, Coonrod S, Hinton BT, et al. Male sexual dysfunction in mice bearing targeted mutant alleles of the PEA3 ets gene. Mol. Cell. Biol. 2000; 20:9337-45.
Background Reference 4:
Oh S, Shin S, and Janknecht R. ETV1, 4 and 5: An oncogenic subfamily of ETS transcription factors. Biochim. Biophys. Acta 2012: 1826:1-12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8535

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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