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Western blot analysis of anti-EXOSC8 Antibody in HL60 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue with EXOSC8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti-EXOSC8 Antibody in HL60 cell line lysates (35ug/lane).

EXOSC8 Antibody

CATALOG NUMBER: 61-502

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EXOSC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-276 amino acids from the C-terminal region of human EXOSC8.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EXOSC8
Additional Names:
Exosome complex component RRP43, Exosome component 8, Opa-interacting protein 2, OIP-2, Ribosomal RNA-processing protein 43, p9, EXOSC8, OIP2, RRP43
Protein Accession Number:
Q96B26
Protein Gi Number:
21759409
Ncbi Gene Id Number:
11340
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EXOSC8 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3'-untranslated regions. It is required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and has a 3'-5' exonuclease activity.
Background Reference 1:
Raijmakers,R., J. Mol. Biol. 323 (4), 653-663 (2002)
Background Reference 2:
Jiang,T., Proc. Natl. Acad. Sci. U.S.A. 99 (8), 5295-5300 (2002)
Background Reference 3:
Raijmakers,R., J. Mol. Biol. 315 (4), 809-818 (2002)
Background Reference 4:
Brouwer,R., J. Biol. Chem. 276 (9), 6177-6184 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-502

List Size:
400 ul

List Price:

$495.00

Datasheet
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