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42-258 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-258 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EYA1 Antibody

CATALOG NUMBER: 42-258

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TDPTAEYSTIHSP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in lysates of cell line HEK293 (calculated MW of 60.7kDa according to NP_742056.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize all isoforms (NP_742057.1; NP_000494.2; NP_742056.1). Reported variants represent identical protein: NP_000494.2, NP_742055.1
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EYA1
Additional Names:
EYA1, eyes absent homolog 1 (Drosophila), BOP, BOR, MGC141875, Eyes absent, Drosophila, homolog of, 1, Melnick-Fraser syndrome, OTTHUMP00000195053, eyes absent 1
Protein Accession Number:
NP_742057.1,NP_000494.2,NP_742056.1
Protein Gi Number:
26667222
Ncbi Gene Id Number:
2138
Background Reference 1:
Orten DJ, Fischer SM, Sorensen JL, Radhakrishna U, Cremers CW, Marres HA, Van Camp G, Welch KO, Smith RJ, Kimberling WJ. Branchio-oto-renal syndrome (BOR): novel mutations in the EYA1 gene, and a review of the mutational genetics of BOR. Human Mutation 2008 Apr 29 (4): 537-44.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-258

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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