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Western blot analysis of extracts of various cell lines, using EYA3 antibody (23-260) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using EYA3 antibody (23-260) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

EYA3 Antibody

CATALOG NUMBER: 23-260

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EYA3 (NP_001981.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
293T
Positive Control 3:
HT-29
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse eye
Positive Control 6:
Rat eye
Predicted Molecular Weight:
Observed: 63kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EYA3
Additional Names:
EYA3,
Ncbi Gene Id Number:
2140
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-260

List Size:
100 uL

List Price:

$436.00

Datasheet
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