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Western blot analysis of extracts of various cell lines, using EZR antibody (13-256) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using EZR antibody (13-256) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using EZR antibody (13-256) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using EZR antibody (13-256) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug EZR antibody (13-256). Western blot was performed from the immunoprecipitate using EZR antibody (13-256) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using EZR antibody (13-256) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

EZR Antibody

CATALOG NUMBER: 13-256

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human EZR (NP_003370.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
IP: 1:50 - 1:100
Positive Control 1:
M21
Positive Control 2:
SW480
Positive Control 3:
U-251MG
Positive Control 4:
293T
Positive Control 5:
Mouse intestine
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 90kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EZR
Additional Names:
Ezrin, VIL2, villin 2 (ezrin), CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, cytovillin, ezrin, villin 2
Ncbi Gene Id Number:
7430
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-256

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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