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45-171 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-171 (3.75ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-171 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-171 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Ezrin Antibody

CATALOG NUMBER: 45-171

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-QDYEEKTKKAER
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80kDa band observed in Human Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Colon and Kidney. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VIL2
Additional Names:
villin 2 (ezrin) , ezrin, VIL2, CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584 , cytovillin, villin 2
Protein Accession Number:
NP_003370.2
Protein Gi Number:
21614499
Ncbi Gene Id Number:
7430
Background Reference 1:
Stanasila L, Abuin L, Diviani D, Cotecchia S. Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling. J Biol Chem. 2006 Feb 17;281(7):4354-63. Epub 2005 Dec 13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-171

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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