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Western blot analysis of lysed extracts from MDA cells untreated or treated with anisomycin using Ezrin (Phospho-Thr567).
Western blot analysis of lysed extracts from MDA cells untreated or treated with anisomycin using Ezrin (Phospho-Thr567).

Ezrin (phospho Thr567) Antibody

CATALOG NUMBER: 79-471

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Ezrin (Phospho-Thr567) antibody was raised against a peptide sequence around phosphorylation site of threonine 567 (Y-K-T (p) -L-R) derived from Human Ezrin.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of Ezrin only when phosphorylated at threonine 567.
Predicted Molecular Weight:
81 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
KDM6A
Additional Names:
UTX, KABUK2, bA386N14.2, MEK3, MKK3, PRKMK3, SKK2, Cytovillin, EZRI, VIL2, Villin 2, p81
Protein Accession Number:
NP_001104547.1
Protein Gi Number:
161702986
Ncbi Gene Id Number:
7403
Background:
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Background Reference 1:
Zhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91Shiue H, et al. (2005) J Biol Chem: 280 (2): 1688-95Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101 (25): 9485-90Fievet BT, et al. (2004) J Cell Biol: 164 (5): 653-9

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-471

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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