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Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Ezrin (Phospho-Tyr353).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin (Phospho-Tyr353).
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Ezrin (Phospho-Tyr353).

Ezrin (phospho Tyr353) Antibody

CATALOG NUMBER: 79-133

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Ezrin (Phospho-Tyr353) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y (p) -E-E) derived from Human EZRIN.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.
Predicted Molecular Weight:
81 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EZR
Additional Names:
CVL, CVIL, VIL2, HEL-S-105, ESR, NR3A1, Cytovillin, EZRI, Villin 2, p81
Protein Accession Number:
NP_001104547.1
Protein Gi Number:
161702986
Ncbi Gene Id Number:
7430
Background:
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Background Reference 1:
Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490. Wang Q, et al. (2003) J Biol Chem 278 (48): 47731-47743. Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96 (13): 7300-7305. Crepaldi T, et al. (1997) J Cell Biol 138 (2): 423-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-133

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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