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Western blot analysis of extracts from K562 cells treated with Na3VO4 using Phospho-Ezrin (Tyr478) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from K562 cells treated with Na3VO4 using Phospho-Ezrin (Tyr478) Antibody. The lane on the right is treated with the antigen-specific peptide.

Ezrin (phospho Tyr478) Antibody

CATALOG NUMBER: 79-960

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Ezrin (phospho Tyr478) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 478 (P-V-Y (p)-E-P) derived from Human Ezrin.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 478.
Predicted Molecular Weight:
70 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EZR
Additional Names:
Cytovillin, EZRI, p81, VIL2, Villin 2
Protein Accession Number:
NP_001104547.1
Protein Gi Number:
161702986
Ncbi Gene Id Number:
7430
Background:
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Background Reference 1:
Murchie R, et al. (2014) Proc Natl Acad Sci U S A 111, 693-8 . Mak H, et al. (2012)BMC Cancer 12, 82. Heiska L, et al. (2011)Oncogene 30, 4953-62 .

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-960

List Size:
0.1 mg

List Price:

$355.00

Out of stock

Datasheet
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