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Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 μg/mL.
Immunohistochemistry of F1Aα in mouse liver tissue with F1Aα antibody at 5 μg/mL.
Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 μg/mL.
Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 μg/ml.<br><br>Green: F1A alpha Antibody (2279) <br> Blue: DAPI staining
Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 μg/mL.
Trial Size

F1A alpha Antibody

CATALOG NUMBER: 2279

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (79%)
Immunogen:
F1A antibody was raised against a 14 amino acid peptide near the carboxy terminus of human F1A.
The immunogen is located within the last 50 amino acids of F1A alpha.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
F1Ab antibody can be used for detection of F1Ab by Western blot at 1 μg/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Positive Control 2:
Cat. No. 1464 - Rat Liver Tissue Lysate
Predicted Molecular Weight:
70 kDa
Purification:
F1A alpha Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
F1A alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
F1A alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FEM1B
Additional Names:
F1A alpha Antibody: F1AA, F1A-ALPHA, FEM1-beta, F1AA, KIAA0396, Protein fem-1 homolog B, FEM1b
Protein Accession Number:
AAF05314
Protein Gi Number:
6175869
Ncbi Gene Id Number:
10116
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
F1A alpha Antibody: Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1β. F1Aalpha/FEM1β is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.
Background Reference 1:
Chan SL, Tan KO, Zhang L, Yee KS , Ronca F, Chan MY, Yu VC. F1Aα, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem. 1999;274(45):32461-8.
Background Reference 2:
Ventura-Holman T, Maher JF. Sequence, organization, and expression of the human FEM1B gene. Biochem Biophys Res Commun 2000;267(1):317-20
Background Reference 3:
Chan SL, Yee KS , Tan KM, Yu VC. The Caenorhabditis elegans sex determination protein FEM-1 is a CED-3 substrate that associates with CED-4 and mediates apoptosis in mammalian cells. J Biol Chem. 2000;275(24):17925-8. (WD0102)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2279

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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