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Western blot analysis of FA2H Antibody in ZR-75-1 cell line lysates (35ug/lane)
Western blot analysis of FA2H Antibody in mouse brain tissue lysates (35ug/lane)
FA2H Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of FA2H Antibody in ZR-75-1 cell line lysates (35ug/lane)

FA2H Antibody

CATALOG NUMBER: 61-669

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Rat
Immunogen:
This FA2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-148 amino acids from the Central region of human FA2H.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FA2H
Additional Names:
Fatty acid 2-hydroxylase, 1---, Fatty acid alpha-hydroxylase, FA2H, FAAH
Protein Accession Number:
Q7L5A8
Protein Gi Number:
74749893
Ncbi Gene Id Number:
79152
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FA2H is a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups.
Background Reference 1:
Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009)
Background Reference 2:
Edvardson, S., et al. Am. J. Hum. Genet. 83(5):643-648(2008)
Background Reference 3:
Uchida, Y., et al. J. Biol. Chem. 282(18):13211-13219(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-669

List Size:
400 ul

List Price:

$495.00

Datasheet
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